Scientific Activity

Brief Description of The Scientific Activity

Biosensors for the detection and characterisation of oxidative damages, conformational and structural modifications of biomolecules upon their interactions with health hazard compounds.

▪ Damages caused to DNA and proteins by reactive oxygen and nitrogen species, heavy metal ions, drugs and radicals arising from redox reactions detected and characterised by voltammetry, electrochemical impedance spectroscopy, atomic force microscopy, ATR-FTIR spectroscopy, HPLC. Determination of biomarkers for biomolecules damages.

 

Detection and characterisation of posttranslational modifications of proteins, especially phosphorylation processes, and protein degradation by proteasome for evaluation of ligands with anti-cancer potential.

▪ Kinases and proteasome represent leading targets for most recent therapeutic intervention in cancer. Assessing their activity and inhibition by electrochemical methods is significant from medical and biochemical points of view, allowing fast screening of ligands with anti-cancer potential.

 

Characterisation of modified electrode surfaces for application to biosensors.

▪ Development of electrochemical biosensors through programmed self-assembling of nanostructured materials and biomolecules (proteins, DNA, etc.) for studying their structures, activities and interactions with (bio)molecules and inhibitory ligands. Biomolecule flexibility and oriented confinement are envisaged. Characterisation of the modified surface by voltammetry, electrochemical impedance spectroscopy, atomic force microscopy.