Molecular Bases of Tumoral Variability: Modifier Genes of The Susceptibility and Evolution of Cancer

Recommended Prerequisites

NA

Teaching Methods

-The classes will be taught totally or partially in English.
-The student must attend the evaluable theoretical classes of the course (13 hours), having read and understood the recommended bibliography previously.
-The student must attend the seminars (12 hours) in which each student will present a published research work related to the course. An evaluable critical dialogue will be established.
To assist in the evaluable practices (4 hours, organized in 2 days), evaluate a backcross, and identify QTL. The practice will take place in a computer classroom.

Learning Outcomes

- To design and organize a model of genetic and phenotypic variability with a mouse backcross. The purpose is to analyze tumor evolutionary and pathophenotypes and subphenotypes of tumor variability between mice. Identification of QTLs.
- To interpret studies of allele-specific mutations in the context of tumor variability for the analysis of intrinsic modifier genes.
- To interpret the manuscript where genetic and molecular determinants of tumor variability are studied.

Work Placement(s)

No

Syllabus

Theoretical classes:
•Lesson 1. Cancer as a complex trait disease
•Lesson 2. Cancer as a complex trait disease
•Lesson 3. Mouse strains for the generation of models of high but simplified genetic variability in the mouse
•Lesson 4. Main biostatistics strategies to integrate variables of different levels and explain complex trai
•Lesson 5. Involvement of extrinsic compartments to the tumor cell in the growth and spread of the tumor.
•Lesson 6. Stem cell and origin and tumor variability.
•Lesson 7. Strategy for identifying genetic and molecular determinants of tumor evolution
Practices
Practice 1. To design and organize a backcross database. To evaluate the distribution of genotypes and tumor pathophenotypes in a backcross cohort that develops breast cancer.
Practice 2: To analyze the distribution of different phenotypes in a backcross cohort of mice genotyped by the Illumina platform. Identification of QTLs.
Seminars
The articles for discussion and presentation

Assessment Methods

Assessment
Exam: 20.0%
Others: 30.0%
Research work: 50.0%

Bibliography

Será disponibilizado durante o curso / Available during the course