Methodologies in Cellular and Molecular Biology

Recommended Prerequisites

Biochemistry, Cell Biology, Molecular Biology.

Teaching Methods

The teaching methodology includes:

Theoretical classes, in which the different methodologies are presented by specialists and their advantages / limitations are discussed;

Theoretical-practical and practical classes, focused on data analysis procedures. 

Learning Outcomes

This course aims to provide students with in-depth knowledge of current and large-scale experimental methodologies and approaches used in the fields of Cellular and Molecular Biology.

Work Placement(s)

No

Syllabus

Fluorescence Microscopy

Basic principles

Hands-on: image acquisition and processing

Flow cytometry and fluorescence-activated cell sorting

Basic principles and applications

Data analysis

Next generation sequencing

Basic principles of NGS and experimental design

RNA-Seq data processing and analysis

Functional Genomics and high-throughput/high-content screening

Gene modulation technologies for high-throughput/high-content screening

Hands-on: image segmentation and automated image analysis

Mass Spectrometry

Basics of MS and LC-MS

Case studies of small molecule targeted analysis and biomarker research

Recombinant protein expression and purification

Cloning strategies and protein expression/purification

Antibody technologies

Antibodies for research/therapy

Recombinant Viral Particles

Production, purification and main applications

Real Time Analysis of Energy Metabolism

Basics of Seahorse technology

Transgenic Animal Models

Strategies and applications,

Head Lecturer(s)

Miguel Luís Cunha Mano

Assessment Methods

Assessment
Mini Tests: 30.0%
Work presentation: 70.0%

Bibliography

H. Lodish et al. (2016) Molecular Cell Biology. W.H. Freeman. 8th edition

B. Alberts et al. (2014) Molecular Biology of the Cell. W.W. Norton & Co. 6th edition

A. Hofmann et al. (2018) Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology. Cambridge University Press. 8th edition.