Advanced Methods of Analysis

Year
1
Academic year
2023-2024
Code
02005856
Subject Area
Chemistry
Language of Instruction
Portuguese
Mode of Delivery
Face-to-face
Duration
SEMESTRIAL
ECTS Credits
5.0
Type
Compulsory
Level
2nd Cycle Studies - Mestrado

Recommended Prerequisites

Not applicable.

Teaching Methods

Lectures, laboratorial demonstrations/laboratorial work.  

Learning Outcomes

The course aims to provide solid knowledge in advanced methods of analysis which find wide application in chemical quality control, in industrial and research laboratories, in environmental monitoring and in Forensic Chemistry.

Work Placement(s)

No

Syllabus

GC-MS, ionization sources, EI. CI: PICI and NICI. HPLC-MS, APCI, APPI, ESI. Mass analyzers. Signal acquisition methods, full scan, SIM. TIC, XIC. Quantitative analysis, internal standard.
Tandem-MS. Operation modes.
Molecular absorption UV/VIS spectroscopy. Diffuse reflectance. UV/VIS microspectroscopy.
Infrared spectroscopy, ATR, DRIFTS. Infrared and Raman microspectroscopies
AAS, flame and graphite furnace atomization. Cold vapor and hydride generation. HR-CS AAS.
ICP-OES. Laser ablation
ICP-MS. Interferences. Reaction and collision dynamic cells. Isotopic dilution method
X-ray fluorescence
Scanning electron microscopy, SEM. SEM-EDX.
Atomic force microscopy.
Electrochemical methods. Voltammetric methods: linear, cyclic and impulse sweep. Potentiometric methods.
Electroanalysis. Electrochemical detectors for continuous flow and hyphenated HPLC systems. Electroanalysis of traces of toxic metal ions.Electrochemical sensors.Applications of electroanalysis in the environment and health.

Head Lecturer(s)

Maria Ermelinda da Silva Eusébio

Assessment Methods

Assessment
Laboratory work or Field work: 30.0%
Exam: 70.0%

Bibliography

Principles of Instrumental Analysis, PF. Holler,  D. Skoog, S. Crouch, , 7th ed.,  Cengage Learning, Inc, Mason, 2017.

Analytical Atomic Spectrometry with Flames and Plasmas, J. A. C. Broekaert,  Wiley-VCH, Weinheim, 2002.

The Essence of Chromatography, C. F. Poole, Elsevier, Amsterdam, 2003..

Fundamentals of Analytical Toxicology, R. J. Flanagan, A. A. Taylor, I. D. Watson, R. Whelpton, Wiley, Chichester, 2008.

Modern Instrumental Analysis,  S. Ahuja, N. Jespersen (eds.),in Comprehensive Analytical Chemistry, D. Barceló (ed.) vol. 47, Elsevier, Amsterdam, 2006.

Electroquímica. Princípios, Métodos e Aplicações C.M.A. Brett e A.M. Oliveira Brett,  Livraria Almedina, Coimbra, 1996.

Electroanalysis C.M.A. Brett e A.M. Oliveira Brett, Oxford University Primers Nº 64, Oxford, 1998.

Laboratory techniques in electroanalytical chemistry P.T. Kissinger e W.R. Heineman Ed., Dekker, NY.

Practical Instrumental Analysis, S. Petrozzi, Wiley-VCH, Weinheim, 2013.