Image Biochemistry

Recommended Prerequisites

NA

Teaching Methods

Theoretical classes: lectures with interaction and debate, using audiovisual media.

Practical classes: students carry out work applying the knowledge learned in the theoretical classes.

Seminars: students present work related to the topics covered, involving self-learning.

Learning activities include debate and inquiry during the various types of classes. The practical/laboratory component is fundamental to this course unit, as it presents real situations related to the professional context.

Learning Outcomes

This course aims at teaching the fundamental concepts that allow for the understanding and critical analysis of the information gathered through imaging of the main tissues and organs, in normal and pathological situations. The metabolic characteristics specific to each tissue and/or organ are approached from a biochemical viewpoint, in order to ease a functional imaging study. Departing from the analysis of a metabolic pathway, the student is lead to understand how to choose the origin of an image that can supply information about that particular metabolic activity. Based on prior knowledge of the metabolism and associated image in normal situations, the student should be able to identify the changes in the image induced by a pathological situation and to suggest new targets for functional imaging.

Work Placement(s)

No

Syllabus

Glycolytic pathway (e.g. FDG, 11C-Glucose)

Fatty acid metabolism (e.g. 11C-Palmitate, 11C-Acetate, and 18F-labelled derivatives)

Oxidative metabolism

Oxidation/reduction pathways and mitochondrial metabolism (e.g. MIBI/Tetrofosmin, Misonidazole derivatives, 11C-Lactate, Oxygen-15)

DNA replication (e.g. 11C-Thymidine and Fluor-18-FLT labelled derivatives)

Amino acid metabolism (e.g. 11C-Methionine, Tyrosine and derivatives, Phenylalanine)

Vitamin B12 metab. (e.g. 111In-DAC)

Phosphocalcic metab. (e.g. bisphosphonates and derivatives)

Bilirubin metab. (e.g. IDA and derivatives)

Thyroid metab. (e.g. iodine and technetium)

Catecholamine metab. (e.g. MIBG, fluorodopa)

Transferrin and iron ligands (e.g. Gallium-67)

Membrane proteins (e.g. GpP Technetium-labelled myocardial agents)

Cell receptors and transporters (Dopamine, Serotonin, Somatostatin)

Transmembrane transport (e.g. Thallium-201)

Cell death pathways (e.g. Annexin-V, Hoechst, Syto-13, Propidium Iodide)

Head Lecturer(s)

Maria Manuela Monteiro Grazina

Assessment Methods

Assessment
Laboratory work or Field work: 10.0%
Report of a seminar or field trip: 15.0%
Resolution Problems: 25.0%
Mini Tests: 50.0%

Bibliography

Lehninger Principles of Biochemistry (8th Edition) 2021

International Edition

Author(s): David L. Nelson; Michael M. Cox

Publisher: Publisher: W.H. Freeman and Company/ Macmillan Learning

 

Biochemistry (9th Edition) 2019

Author(s): Jeremy M. Berg, Lubert Stryer, John Tymoczko, Gregory Gatto

Publisher: W.H. Freeman and Company/ Macmillan Learning

 

Biomedical Imaging: Applications and Advances 2014

Editor(s): Peter Morris

Publisher: Elsevier

 

Essentials of Nuclear Medicine and Molecular Imaging 2018

Author(s): Fred A. Mettler, Jr. and Milton J. Guiberteau. 7th Edition.

Publisher: Elsevier

 

Nuclear Medicine and Molecular Imaging: Technology and Techniques 2022

Author(s): David Gilmore, Kristen M. Waterstram-Rich

Editor(s): Kristen M. Waterstram-Rich, David Gilmore. 9^th edition

Publisher: Elsevier Health Sciences Division

 

Artigos científicos indicados nos materiais de apoio.

Protocolos fornecidos nos materiais de apoio.