Experimental Embryology
1
2021-2022
02001971
Biodiversity and Biotechnology
Portuguese
Face-to-face
SEMESTRIAL
6.0
Elective
2nd Cycle Studies - Mestrado
Recommended Prerequisites
General knowledge of genetics, plant physiology and molecular biology.
Teaching Methods
Two-week intensive course with theoretical and practical classes. During the two weeks the students will grouped (3 – 5) and will develop a practical work related with one particular subject of plant embryogenesis. The results will be discussed with the professor and colleagues and, by the end of the course students will write a report about the experiments and will present and discuss their work.
Learning Outcomes
1. Understanding capacity:
To be able to understand concepts related with embryogenesis and to relate these concepts with general plant development and with practical applications of areas like agriculture and plant biotechnology
2. Ability to solve practical problems:
To apply the concepts and methods learnt on this course to solve practical issues.
3. Communication:
Be able to analyse scientific information and to transmit it in a clear and concise way either in oral or written form.
4. Self learning:
To be able and autonomous in the preparation and analysis of scientific experiments. Show the capacity to interpret scientific data according to the backgrounf information available in the literature.
Work Placement(s)
NoSyllabus
Sexual reproduction. Double fertilization in angiosperms
Zygote and embryogenesis
Suspensor and endosperm
Physiological and molecular mechanisms involved on embryo development
Arabidopsis as a model to study embryogenesis
Embryo mutants
Genes and embryogenesis
The Fucus zygote
Non-zygotic embryogenesis
Different types on non-zygotic embryogenesis occurring in plants
Non-zygotic embryogenesis experimentally induced
Apomixis.
Somatic embryogenesis
Types of somatic embryogenesis
Cytological, physiological and molecular characterisation of somatic embryogenesis
Auxins and stress conditions on somatic embryogenesis induction
Maturation and germination of somatic embryos
Pollen embryogenesis and gynogenesis
Totipotency of microspores and pollen grains
Pollen embryogenic pathways
haploid plants
Pollen plants production and its applications in biotechnology
Embryo rescue and culture
Isolation and culture of zygotic embryos
In vitro fertilisation
Hybrids
Embryogenesis and plant breeding
Head Lecturer(s)
Jorge Manuel Pataca Leal Canhoto
Assessment Methods
Assessment
Laboratory work or Field work: 50.0%
Exam: 50.0%
Bibliography
-Buchanan, B.B., Gruissem, W. & Jones, R.L., 2000. Biochemistry and Molecular Biology of Plants. Amer. Soc. Plant Physiol., Rockville.
Hodson, M.J. & Bryant J.A., 2012. Functional Biology of Plants. Wiley-Blackwell, Oxford.
-Lindsey, K., 2004. Polarity in Plants. Blackwell Publishing and CRC Press, Oxford.
- Copeland, L.O. & Mcdonlad, M.B., 2001. Seed Science and Technology (4ª ed.). Kluwer Academic Publishers, Dordrecht.
Palme, K., 1994. Signals and Signal Transduction Pathways in Plants. Kluwer Academic Publishers, Dordrecht.
-Raghavan, V., 1997. Molecular Embryology of Flowering Plants. Cambridge University Press, Cambridge.
Taiz, L. & Zeiger, E., 2010. Plant Physiology (5ª ed.). Sinauer Associates, Inc., Publishers, Sunderland.
-Thorpe, T.A., 1995. In Vitro Embryogenesis in Plants. Kluwer Academic Publishers, Dordrecht.
-Trigiano, R.N. & Gray, D.J., 2005. Plant Development and Biotechnology. CRC Press, Boca Raton.