Experimental Techniques I

Recommended Prerequisites

NA

Teaching Methods

The teaching of this unit uses the expository method, problem solving and task development. In PL classes students will be divided into small groups (maximum of 3 students per group). Each student will have to perform the tasks inherent to each technique.

Learning Outcomes

This curricular unit aims to provide students with skills on the use of different laboratory techniques, important in the context of experimental research. The student will develop skills to select the most appropriate techniques for each particular situation, on theoretical knowledge (understand, follow and correlate the knowledge of the subject). It should also develop the capacity to acquire knowledge autonomously, to integrate knowledge, and to have critical ability.

Work Placement(s)

No

Syllabus

The student will pass through various techniques of molecular and cellular biology, starting from cell cultures:

- Introduction to cell culture. Advantages, disadvantages, care to have.

- Tests to assess cell proliferation and viability

- Western blot, ELISA and PCR

- Flow cytometry

- Metabolism, Proteomics and Lipidomics.

Head Lecturer(s)

Marilia Assunção Rodrigues Ferreira Dourado

Assessment Methods

Assessment
Continuous assessment: 50.0%
Exam: 50.0%

Bibliography

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. R. Ian Freshney

Cell Viability Protocols and Applications Guide, 2011

Quantitative Assessment of Cell Quality, Viability and Proliferation, 2012

Western_Blotting_ScienceGE2booklet_MedRes, 2015

Western Blotting Handbook, Pierce, 2012

Western Blotting Principles and Methods, GE

ELISA Handbook,

The ELISA Guidebook, 2002

PCR-application-manual-3rd-ed,

Flow cytometry: basic principles and applications, 2013

Introduction to Flow Cytometry: A Learning Guide

Metabolomics, 2012

Livros e artigos científicos de revistas sobre anatomia e fisiologia comparadas / Scientific books and papers from journals about compared anatomy and physiology