Instrumental Methods in Clinical Laboratory

Recommended Prerequisites

The fundamental basic knowledge must be acquired in Course Units of physical chemistry and analytical chemistry with an emphasis on instrumental analytical and bioanalytical methods. Also important are laboratory skills and abilities.

Teaching Methods

The course is based on two types of classes:

i) Theoretical classes, where the different course subjects are presented and discussed;

ii) Theoretical-practical classes, intended for clarification, practical application and sedimentation of the

concepts presented in the lectures, through different strategies, as indicated in the program.

Learning Outcomes

The main objectives of this course are to acquire knowledge, competencies, and skills, in an integrated manner, of the principles of instrumental analysis and automation in the Clinical Analysis Laboratory. Thus, in the end, students should i) have acquired a broad knowledge of the basic principles of operation of instrumental techniques and analytical methodologies used most commonly in clinical laboratories;

ii) adquiram os conhecimentos sobre os métodos de calibração e os conceitos básicos de instrumentação e desempenho analítico;

iii) have acquired the basics of clinical laboratory automation and the various types of automated equipment commonly used.

iv) demonstrate competence and skills in the collection of specimens for analysis.

Work Placement(s)

No

Syllabus

PROGRAMME

Introduction

Preanalysis

Instrumental Methods of Analysis

Atomic and Molecular Spectroscopy

Chemiluminescence

Electroanalysis

Chromatography-Mass Spectrometry

Immunochemical Methods

Molecular Methods

Chemical Sensors and Biosensors

Automation in Clinical Laboratory

Collection of specimens for analysis in collaboration with Escola Superior de Enfermagem de Coimbra)

Head Lecturer(s)

João Manuel Martins Leitão

Assessment Methods

Assessment
Exam: 100.0%

Bibliography

-Skoog, D.A., Holler, E. J., Crouch, S. R., Principles of instrumental analysis. 7th ed. Thomson Brooks/Cole. 2017.

-Harris, D.C. Quantitative chemical analysis. 9th ed. New York: W. H. Freeman. 2018.

-Henry’s Clinical Diagnosis and Management by Laboratory Methods (22nd Edition), McPherson and Pincus, Eds. Elsevier Saunders (2011).

-Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 7th (2014) Fundamentals of Clinical Chemistry (Tietz) 7th Edition by Carl A. Burtis and David E. Bruns.

-Treas, L.; Wilkinson, J. (2014). Basic Nursing Concepts Skills & Reasoning. Philadelphia, EUA: F.A. Davis Company