Methods and Techniques in Cytology and Physiology

Recommended Prerequisites

None with the exceptions of basic concepts of Biology, Chemistry and Physics.

Teaching Methods

Theoretical-practical classes during the semester concerning the principles and applications of the methods and techniques used in Citology and Physiology.

Learning Outcomes

The aim is to provide students with: i) a consistent and actualized knowledge of the methods and techniques used in Citology and Physiology, ii) their fundamental principles and practical applications.

Work Placement(s)

No

Syllabus

1) Light Microscope: principles and practical.
2) Phase Contrast, Dark Field and Fluorescence Microscopes: principles and practical.
3) Transmission and Scanning Electron Microscopes: principles and visite to the Laboratory of Electron Microscopy. Interpretation de Micrographs.
4) Techniques of praparation of the cells and tissues for Optical and Electron Microscopy - the Histological Technique.
5) Citochemistry.
6) Histoenzimology.
7) Chromatography.
8) In vitro cultures.

Head Lecturer(s)

António Xavier de Barros e Cunha Pereira Coutinho

Assessment Methods

Assessment
Laboratory work or Field work: 20.0%
Exam: 80.0%

Bibliography

Bandhari, N. N. 1997. Staining Techniques: a manual. Ultimate Printers, New Delhi.

 

Bozzola, J. J. & Russel, L. D. 1998. Electron Microscopy (2nd edition). John and Bartlett Publishers, Sudbury, Massachussets.

 

Hopkins, W. G. 1999. Introduction to Plant Physiology (2nd edition). John Wiley and Sons, Inc., New York, Chichester, Weinheim, Brisbane, Singapore, Toronto.

 

Prado, C. H & Casali, C. A. 2006. Fisiologia Vegetal . Práticas em Relações Hídricas, Fotossíntese e Nutrição Mineral. Editora Manole Ltda., Tamboré.

 

Textos de Apoio elaborados pelos professores da unidade curricular.