Cell and Molecular Biology

Recommended Prerequisites

Basic knowledge on chemistry, biology and mathematics.

Teaching Methods

The teaching methodologies combine
1) Lectures in which the concepts are presented when possible on the basis of the original experimental evidence that supports them;
2) Laboratory work. For these classes, the students are encouraged to work autonomously, and are asked to prepare solutions and the biologic material, to collect data and to interpret data on their own.

Learning Outcomes

The aim of this course is that the students acquire essential knowledge on biology, at the molecular and cellular levels. The students will know the molecular composition of the cell, and the cellular function of the different macromolecules; in addition, they will learn about the different cellular organelles and will be able to relate their structure to the fulfilled function. The students will also come in contact with the potential of novel cellular therapies, for example involving stem cells. It is expected that the students will understand the molecular bases of the hereditary succession, and that they understand the flow of genetic information from genes to proteins and cells; in addition, the mechanisms that regulate gene expression will be explores. Finally, the students will learn the principles that underlie recombinant DNA technology, genomics and the possibility to create genetically modified animals.

Work Placement(s)

No

Syllabus

1. The cell: basic life unit
a) Prokaryotic and eukaryotic cells
b) Methods in cell biology
c) Cellular organization
c.1. Information processing organelles
c.2. The endomembrane system: the endoplasmic reticulum, the Golgi complex and lysosomes: intracellular protein traffic and targeting
c.3. Energy processing organelles: mitochondria and chroloplasts.
c.4. The cytoskeleton: cell shape and movement
c.5. Cell surfaces and junctions

2. Cell communication and homeostasis
a) Cell communication and receptors
b) Second messengers
c) Regulation by hormones
d) Cell homeostasis
e) The cell cycle

3. Information pathways and inheritance
a) The organization of eukaryotic genomes; DNA structure
b) DNA replication and repair
c) From gene to protein
d) Transcriptional and post-transcriptional regulation of gene expression
e) DNA technology

4. Interface between molecular cell biology and new biomedical therapies.

Assessment Methods

Assessment
Report of a seminar or field trip: 10.0%
Laboratory work or Field work: 10.0%
Synthesis work: 15.0%
Exam: 65.0%

Bibliography

• Life: The Science of Biology. David Sadava et al. (2014) Sinauer Associates, Inc (10ª Edição)
• Campbell Biology. Jane B Reece et al. (2014)  Pearson (10ª Edição)
• The Cell - A Molecular Approach, G.M. Cooper & Robert E. Hausman (2015) Sinauer Associates (7ª Edição).
• Molecular Cell Biology, Harvey Lodish et al. (2016) W.H. Freeman and Company (8ª Edição).
• Cell and Molecular Biology: Concepts and Experiments, Gerald Karp, Janet Iwasa, Wallace Marshall (2015 - 8ª Edição).
• Biologia Celular e Molecular, Carlos Azevedo, Claudio E Sunkel (2012) LIDEL-edições técnicas, lda. (5ª Edição).