Laboratories of Analytical Biochemistry

Recommended Prerequisites

Prior attendance of a laboratorial course.

Teaching Methods

Students prepare each practical class based on theoretical information (TP classes),  protocol, and included bibliography. Students work in groups of 2-4 autonomously, but supervised. The results are recorded in the laboratory notebook, analyzed, interpreted and discussed critically.

Learning Outcomes

The course is designed for acquisition of skills and attitudes needed for the work in a research or biochemical analysis laboratory. The course aims at the understanding and implementation by the students of technical and laboratory processes and their use in biochemistry, in a rigorous, precise, and productive way.

Work Placement(s)

No

Syllabus

Performance of Analytical Biochemistry work using the main techniques and methods of this area. It will be considered a set of works involving: a. spectroscopic methods (eg UV/visible absorption spectrophotometry); b. methods of isolation and separation (eg, partition chromatography), c. quantification techniques (eg volumetry).

Head Lecturer(s)

Ângelo José Ribeiro Tomé

Assessment Methods

Assessment
Laboratory work or Field work: 20.0%
Exam: 80.0%

Assessment
Mini Tests: 20.0%
Exam: 80.0%

Assessment
Mini Tests: 20.0%
Laboratory work or Field work: 20.0%
Exam: 60.0%

Bibliography

D. Holme and H. Peck (1998) Analytical Biochemistry, 3 rd ed., Pearson Education Ltd, England.

K. Wilson and J. Walker (2010) Principles and Techniques of Biochemistry and Molecular Biology, 7 th ed., Cambridge University Press, England.

R. Katoch (2011) Analytical Techniques in Biochemistry and Molecular Biology, Springer, New York, USA.