Bacteriology and Bacterial Analyses

Recommended Prerequisites

General Microbiology, Biochemistry, Organic Chemistry, Portuguese.

Teaching Methods

The lectures are presented on the classical model, supported with summarized and illustrative presentations in Power- Point. Questions are formulated requiring that students use concepts previously acquired, thus correcting the limitations of dogmatic presentation. Theoretical subjects are essential for interpreting the results obtained in
the laboratory classes, and are always taught before the laboratory class. In laboratory classes, the teacher performs initially the procedure. Students work individually. The analysis of data is performed in group.

Learning Outcomes

The student should know the major groups of bacteria, their characteristics and infections associated with each group, the mechanism of action of antibiotics and their antibacterial spectrum, mechanisms of antibiotic resistance and the genetic basis of spread of resistance, and the methods used to assess the antibiotic susceptibility.

In Practical Laboratory teaching, the student should acquire knowledge on identification of the major groups of pathogenic bacteria and in the laboratorial methods for their identification, to perform a PCR and understand how it can be applied to the identification of bacteria, to evaluate the susceptibility to antibiotics, to screen for beta-lactamases phenotypically and to interpret the results.

Work Placement(s)

No

Syllabus

THEORETICAL

• Introduction to Bacteriology
• Etiological agents: Enterobacteriaceae; Non-fermenter Gram negative bacilli; Staphylococcus spp .; Streptococcus spp .; Enterococcus spp .; Neisseria sp; Haemophilus sp; Mycobacterium tuberculosis. Habitat, morphology, physiology, virulence factors, infections, bacterial identification.
• Antimicrobial agents: mechanisms of action, spectrum of activity, mechanisms of resistance. Quantitative and qualitative susceptibility methods.
• Molecular epidemiology of bacterial resistance: conjugation, transformation, transduction.

LABORATORY

• Isolation and bacterial identification by phenotypic methods: Enterobacteriaceae, Non-fermenter Gram negative bacilli, Staphylococcus spp.; Streptococcus spp.; Enterococcus spp.; Neisseriae sp.; Haemophilus sp.
• Identification of Mycobacterium tuberculosis by PCR
• Evaluation of antibacterial susceptibility.
• Phenotypic detection of broad spectrum β-lactamases, AmpC and metallo-β-lactamase.

Head Lecturer(s)

Gabriela Conceição Duarte Jorge Silva

Assessment Methods

Assessment
Exam: 100.0%

Bibliography

Microbiologia Médica, Ed Barroso, H, Meliço-Silvestre, A, Taveira, N, vol. 1, 1ª ed Lidel 2014

Microbiology -a Human Perspective Ed. Nester, E. W., Roberts, C. E., Pearsall, N. N. Anderson, D. G. and Nester, M. T. 5^th ed. McGraw Hill, Inc. 2007

Medical Microbiology, P. R. Murray, K. S. Rosenthal, M. A. Pfaller, 5^Th ed. Elsevier Mosby 2005.

 

Manual de Antibióticos Antibacterianos. JC Sousa. Edições Universidade Fernando Pessoa 2005

 

Microbiologia. Wanda Ferreira e J. C. Sousa. Volume 1. Lidel 1998