Bioanalytical Chemistry

Recommended Prerequisites

Not applicable.

Teaching Methods

In the curriculum the discipline Bioanalytical Chemistrycorresponds to 6 credit units and a 14-week semester, the total number of predicted classes will be 28 lectures (2 1/2 hours per week) and 14 theoretical-practical classes (2 hours per week). Teaching methods consist of classes: theoretical and theoretical-practical, in order to attain the aim global learning.

Learning Outcomes

The aim of the discipline of Bioanalytical Chemistry is to give students the opportunity to gain a solid training in analytical chemistry to understand the importance of chemistry and in the context of Medicinal Chemistry. In addition, this course will contribute to a greater understanding of the reactions, and the quantification of biological compounds and drugs. The objectives are discussed in detail in the program.

Work Placement(s)

No

Syllabus

I-Bioelectroanalysis. Electric potential of biomolecules. Electron transfer reactions in biological systems. Electron transfer in interfaces. Voltammetric techniques and electrochemical methods, electrochemical cells, electrodes and electrolytes.
Development of electrochemical biosensors: amperometric or potentiometric - clinical applications and in vivo implantation, miniaturization, and determination of metal ions and toxic pesticides.
Electrochemical detectors – HPLC.
Electrochemistry of drugs. Quantification of DNA damage due to anticancer drugs using electrochemical methods
II-Spectroscopic methods: UV-visible, fluorescence, and infrared.
III- High performance liquid chromatography: RPLC, ion chromatography, affinityand size exclusion. Gas chromatography
IV- Gel and Capillary electrophoresis. Zone electrophoresis. Isoelectric focusing.
V- Mass spectrometry.Ionization sources. Mass analyzers. Detectors. Spectra interpretation. Hyphenated techniques.

Head Lecturer(s)

Ana Maria Coelho Ferreira de Oliveira Brett

Assessment Methods

Evaluation
Exam: 100.0%

Bibliography

1. C.M.A. Brett e A.M. Oliveira Brett, Electroquímica. Princípios, Métodos e Aplicações, Livraria Almedina, Coimbra, 1996.
2. C.M.A. Brett e A.M. Oliveira Brett, Electroanalysis, Oxford University Primers n.º 64, Oxford, 1998.
3. P.T. Kissinger e W.R. Heineman Ed., Laboratory techniques in electroanalytical chemistry, Dekker, NY, 1996.
4. A.E.G. Cass, Biosensors. A Practical Approach, IRL Press, OUP, Oxford, 1990.
5. J.P. Hart, Electroanalysis of Biologically Important Compounds, Ellis Horwood, Chichester, 1990.
6. A. Marz, N. Pamme, D. Iossifidis, Bioanalytical Chemistry, Imperial College Press, London, 2004.
7. D. A. Skoog, F. J. Holler, S. R. Crouch, Principles of Instrumental Analysis, Thomson Brooks/Cole, 2007.
8. C. F. Poole, The Essence of Chromatography, Elsevier Science, Amsterdam, 2003.
9. Modern Instrumental Analysis,S. Ahuja, N. Jespersen (eds.), in Comprehensive Analytical Chemistry, D.
Barceló(ed.) vol. 47, Elsevier, Amsterdam, 2006.