Clinical Bacteriology

Recommended Prerequisites

Microbiology, Biochemistry, Molecular and Cellular Biology

Teaching Methods

The theoretical lessons are based in the classical model of knowledge transmission, using audio-visual presentation to illustrate the various subjects explained. During the class questions are asked in order to stimulate student participation and assess the learning outcomes. In this way dogmatic exposition limitations will be corrected allowing a pedagogic interaction between teacher and student.

In laboratory classes, students execute the works proposed in the laboratorial program.

Learning Outcomes

The student must acquire knowledge about clinical bacteriology, and antimicrobials as well as evaluation of bacterial susceptibility to these agents. Objectives are:
a) To study the major groups of bacteria, and respective infections with reference to mechanisms of pathogenesis and virulence factors
b) Provide knowledge about the morphology and physiological characteristics of the infectious agents that are important for laboratory identification
c) Application of theoretical knowledge in practical classes
d) To give the student the ability to stain, isolate and identify the major groups of bacteria commonly found in clinical samples
e) Check the student's ability to learn and perform the susceptibility testing, choosing the antibiotics for the bacteria under study
f) To give the student the ability to interpret laboratory data, encouraging critical thinking and helping to solve problems
g) Encourage the active participation of students in lectures and laboratory.

Work Placement(s)

No

Syllabus

Program theoretical

Introduction to Clinical Bacteriology

Enterobacteriaceae, bacilli Gram negative non-fermentator; Staphylococcus sp, Streptococcus sp; Neisseria sp; Haemophilus sp, Mycobacterium sp.,Campylobacter sp e Helicobacter pylori: general characteristics; mechanisms of pathogenesis, biological samples, laboratory diagnosis and susceptibility to antimicrobials.

Antimicrobial agents: mechanisms of action and resistance, structure-activity relationships. Methods for the determination of the susceptibility: qualitative and quantitative methods. Reference to phenotypic search of resistance mechanisms to beta-lactams and aminoglycosides.

Program Laboratorial

Isolation and identification of enterobacteria.

Isolation and identification of bacilli Gram negative nom-fermentor.

Isolation and identification of Staphylococcus sp.

Isolation and identification of Streptococcus sp.

Head Lecturer(s)

Gabriela Conceição Duarte Jorge Silva

Assessment Methods

Assessment
Other: Practical (30%) – written test (15%) and a laboratory evaluation (15%): 30.0%
Exam: 70.0%

Bibliography

Microbiology -a Human Perspective Ed. Nester, E. W., Roberts, C. E., Pearsall, N. N. Anderson, D. G. and Nester, M. T. 5th ed. McGraw Hill, Inc. 2007

Microbiologia. Wanda Ferreira e J. C. Sousa. Volume 1. Lidel 1998

Medical Microbiology, P. R. Murray, K. S. Rosenthal, M. A. Pfaller, 5Th ed. Elsevier Mosby 2005.

Manual de Antibióticos Antibacterianos .JC Sousa. Edições Universidade Fernando Pessoa 2005

Textbook of Diagnostic Microbiology, C. R. Mahon, G. Manuselis, 2nd edition, W.B. Saunders Company 2000

Microbiologia Médica,Helena Barroso, António Meliço-Silvestre e Nuno Taveira, Volume 1 Lidel Ed Técnicas 2014