Clinical Bacteriology

Recommended Prerequisites

Microbiology, Biochemistry, Molecular and Cellular Biology

Teaching Methods

The theoretical lessons are based in the classical model of knowledge transmission, using audio-visual presentation to illustrate the various subjects explained. During the class questions are asked in order to stimulate student participation and assess the learning outcomes. In this way dogmatic exposition limitations will be corrected allowing a pedagogic interaction between teacher and student.

In laboratory classes, students execute the works proposed in the laboratorial program.

Learning Outcomes

The student must acquire knowledge about clinical bacteriology, and first of all:

Know the major groups of bacteria, and respective infections with reference to mechanisms of pathogenesis and virulence factors and the various antibacterial agents used for the therapy. Provide knowledge about the morphology and physiological characteristics of the infectious agents that are important for laboratory identification

In a 2nd phase, linking previous knowledge to apply them to the laboratory diagnosis of infections of various body systems, in which the student should know and understand how to approach clinical specimens, to cultivate and identify bacteria responsible for the various etiologies, and interpretation of the susceptibility to antibacterial agents The knowledge will allow the acquisition of skills in the area of bacterial laboratory diagnostics. This will be critical to the performance of future clinical analyst, as a health professional.

Work Placement(s)

No

Syllabus

Theoretical program

1. Introduction to Clinical Bacteriology

2. Enterobacteriaceae family; Gram-negative non-fermenting bacilli; Staphylococcus sp; Streptococcus sp; Enterococcus sp; .Neisseria sp; Haemophilus sp; Mycobacterium sp .: general characteristics; pathogenesis mechanisms and therapy.

3. Antimicrobial agents: mechanisms of action and resistance, and spectrum of activity. Quantitative and qualitative methods for the determination of susceptibility.

 

Laboratory Program

1. Isolation and identification of Enterobacteriaceae, Gram-negative non-fermenting bacilli, Staphylococcus sp, Streptococcus sp and Enterococcus sp.

Head Lecturer(s)

Olga Maria Antunes Rodrigues Carvalho Cardoso

Assessment Methods

Assessment
Laboratorial /Practical (30%) – twritten test (15%) and a laboratory evaluation (15%). : 30.0%
Exam: 70.0%

Bibliography

Microbiology -a Human Perspective Ed. Nester, E. W., Roberts, C. E., Pearsall, N. N. Anderson, D. G. and Nester, M. T. 8th ed. McGraw Hill, Inc. 2016

Sherris’s Medical Microbiology: An Introduction to Infectious Diseases, K. J. Ryan & C. G. Ray. 6th ed. 2014. McGraw-Hill

Medical Microbiology, P. R. Murray, K. S. Rosenthal, M. A. Pfaller, 8Th ed. Elsevier Mosby 2016.

Manual de Antibióticos Antibacterianos .JC Sousa. Edições Universidade Fernando Pessoa 2005

Textbook of Diagnostic Microbiology, C. R. Mahon, G. Manuselis, 5th edition, W.B. Saunders Company2015

Manual of Clinical Microbiology, P R Murray, E J Baron, J H Jorgensen, M L Landry, M A Pfaller. 9th ed.. ASM press, Washington 2007

Microbiologia Médica, Helena Barroso, António Meliço-Silvestre e Nuno Taveira, Volume 1 Lidel Ed Técnicas 2014