Instrumental Methods of Analysis I
2
2018-2019
01550141
Chemical and Physical Sciences
Portuguese
Face-to-face
SEMESTRIAL
5.0
Compulsory
1st Cycle Studies
Recommended Prerequisites
The fundamental base of knowledge must be acquired in the disciplines of physics, chemistry and analytical chemistry. It is also essential to have a good understanding of mathematical calculus and statistics. The inorganic chemistry, organic and physical-chemistry knowledge are complementary.
Teaching Methods
In the theoretical teaching the traditional lecture system of teaching is followed. The teacher delivers formal lectures to transmit knowledge by using PowerPoint presentations. The students work in groups, guided by the teacher through the experimental process, in a cooperative learning base. In the classroom the students are encouraged to participate actively in practical and laboratorial classes.
Learning Outcomes
The focus of this curricular unit is the study of the instrumental methods of analysis most used and most relevant in the field of the Pharmaceutical Sciences. It’s expected that the students
Understand the chemical and physical principles underlying the instrumental methods of analysis studied
Understand the potential and limitations of the instrumental methods studied in solving analytical problems
Acquire the basics of analytical instrumentation and its analytical performance characteristics
Select the most appropriate analytical methods to analyse a given analyte and matrix
Learning to operate the analytical instruments, calibrate and analyse the data from the experiments
With the practical and laboratory classes it’s expected that the students will be able to
Execute with skill and efficiency the lab work
Follow rules of safety and environmental protection
To analyse and present laboratory results accurately, clearly and concisely
Prepare reports and laboratory notebooks.
Work Placement(s)
NoSyllabus
Introduction to the study of Instrumental Methods of Analysis
Electrochemical methods
Potentiometry
Condutimetry
Voltammetry
Spectroscopic methods
Molecular Ultraviolet and Visible absorption spectroscopy
Molecular Fluorescence spectroscopy
Atomic absorption and emission spectroscopy
Chromatographic methods
Liquid chromatography
Gas chromatography
Electrochemical methods
Potentiometry: Direct (pH and fluoride) and indirect determinations (acid/base titrations)
Condutimetry: Determination of cell constant, direct (conductance and specific conductance) and indirect determinations (acid/base titrations)
Voltammetry: Characterization of a redox system and quantitation
Spectroscopic methods
UV-Visible spectrophotometry: Register of a spectra and quantification
Fluorescence spectrophotometry: Evaluation of the characteristics of a Fluorescent chemical system and quantification
Atomic absorption and emission: Quantification
Chromatographic methods
HPLC: Identification and quantification.
Head Lecturer(s)
Rui Manuel Silva Gomes Barbosa
Assessment Methods
Assessment
Other: Theoretical-practical test: 25.0%
Exam: 75.0%
Bibliography
Skoog, D.A. et al., Principles of instrumental analysis. 6th ed. Brooks/Cole. 2007.
Harris, D.C. Quantitative chemical analysis. 8th ed. New York: W. H. Freeman. 2011.
Willard, H. H. et al. Instrumental Methods of Analysis. Instrumental methods of analysis. 7th ed. Belmont: Wadsworth Publishing Company. 1988.
Evans, A., Potentiometry and Ion Selective Electrodes, John Wiley & Sons, ACOL, 1987
Riley, Tom; Watson, Arthur, Polarography and other voltametric methods, John Wiley. 1987.
Thomas, Michael J.K. Ultraviolet and visible spectroscopy. 2nd ed. Chichester [etc.]: John Wiley. 1996.
Rendell, David. Fluorescence and phosphorescence spectroscopy. Chichester [etc.]: John Wiley & Sons, 1987.
Evans, E. H. et al., An Introduction to Analytical Atomic Spectrometry. Chichester [etc.]: John Wiley & Sons. 1998.
Lindsay, S. High performance liquid chromatography. 2nd ed. Chichester [etc.]: John Wiley & Sons. 1998