Instrumental Analysis

Recommended Prerequisites

The fundamental base of knowledge must be acquired in the disciplines of physics, chemistry and analytical chemistry. It is also essential to have a good understanding of mathematical calculus and statistics. The inorganic chemistry, organic and physical-chemistry knowledge are complementary.

Teaching Methods

In the theoretical teaching the traditional lecture system of teaching is followed. The teacher delivers formal lectures to transmit knowledge by using PowerPoint presentations. The students work in groups, guided by the teacher through the experimental process, in a cooperative learning base. In the classroom the students are encouraged to participate actively in practical and laboratorial classes.

Learning Outcomes

The focus of this curricular unit is the study of the instrumental methods of analysis most used and most relevant in the field of the Bioanalytical Sciences. It’s expected that the students:

-Understand the chemical and physical principles underlying the instrumental methods of analysis studied.

-Understand the potential and limitations of the instrumental methods studied in solving analytical problems;

-Acquire the basics of analytical instrumentation and its analytical performance characteristics;

-Select the most appropriate analytical methods to analyse a given analyte and matrix:

-Learning to operate the analytical instruments, calibrate the data from the experiments:

With the practical and laboratory classes it’s expected that the students will be able to:

-Execute with skill and efficiency the lab work;

-Follow rules of safety and environmental protection;

-To analyse and present laboratory results accurately, clearly and concisely;

-Prepare reports and laboratory notebooks.

Work Placement(s)

No

Syllabus

Introduction to the study of Instrumental Methods of Analysis

Electrochemical methods

Potentiometry

Condutimetry

Voltammetry

Spectroscopic methods

Molecular Ultraviolet and Visible absorption spectroscopy

Molecular Fluorescence spectroscopy

Atomic absorption and emission spectroscopy

Chromatographic methods

Liquid chromatography

Gas chromatography

Electrochemical methods

Potentiometry: Direct (pH and fluoride) and indirect determinations (acid/base titrations)

Condutimetry: Determination of cell constant, direct (conductance and specific conductance) and indirect determinations (acid/base titrations)

Voltammetry: Characterization of redox systems and quantitation

Spectroscopic methods

UV-Visible spectrophotometry: Register of a spectra and quantification

Fluorescence spectrophotometry: Evaluation of the characteristics of a Fluorescent chemical system and quantification

Atomic absorption and emission: Quantification

Chromatographic methods

HPLC: Identification and quantification

Head Lecturer(s)

Rui Manuel Silva Gomes Barbosa

Assessment Methods

Assessment
Other: Theoretical-practical test: 25.0%
Exam: 75.0%

Bibliography

Skoog, D.A. et al., Principles of instrumental analysis. 6^th ed. Brooks/Cole. 2007.

Harris, D.C. Quantitative chemical analysis. 8^th ed. New York: W. H. Freeman. 2011.

Willard, H. H. et al. Instrumental Methods of Analysis. Instrumental methods of analysis. 7^th ed. Belmont: Wadsworth Publishing Company. 1988.

Evans, A., Potentiometry and Ion Selective Electrodes, John Wiley & Sons, ACOL, 1987

Riley, Tom; Watson, Arthur, Polarography and other voltametric methods, John Wiley. 1987.

Thomas, Michael J.K. Ultraviolet and visible spectroscopy. 2nd ed. Chichester [etc.]: John Wiley. 1996.

Rendell, David. Fluorescence and phosphorescence spectroscopy. Chichester [etc.]: John Wiley & Sons, 1987.

Evans, E. H. et al., An Introduction to Analytical Atomic Spectrometry. Chichester [etc.]: John Wiley & Sons. 1998.

Lindsay, S. High performance liquid chromatography. 2nd ed. Chichester [etc.]: John Wiley & Sons. 1998