Instrumental Analysis

Recommended Prerequisites

he fundamental base of knowledge must be acquired in the disciplines of physics and analytical chemistry. It is also essential to have a good understanding of mathematical calculus and statistics. The inorganic chemistry, organic and physical-chemistry knowledge are complementary.

Teaching Methods

In theoretical teaching, the traditional lecture system of teaching is followed. The teacher delivers formal lectures to transmit knowledge by using PowerPoint presentations. The students work in groups, guided by the teacher through the experimental process, in a cooperative learning base. In the classroom, the students are encouraged to participate actively in practical and laboratory classes.

Learning Outcomes

The focus of this curricular unit is the study of the instrumental methods of analysis most used and most relevant in the field of Pharmaceutical Sciences. It’s expected that the students:

-Understand the chemical and physical principles underlying the instrumental methods of analysis studied.

-Understand the potential and limitations of the instrumental methods studied in solving analytical problems;

-Acquire the basics of analytical instrumentation and its analytical performance;

-Select the most appropriate analytical methods to analyse a given analyte and matrix:

-Learning to operate the analytical instruments, calibrate and analyse the data from the experiments:

With the practical and laboratory classes it’s expected that the students will be able to:

-Execute with skill and efficiency the lab work;

-Follow rules of safety and environmental protection;

-To analyse and present laboratory results accurately, clearly and concisely;

-Prepare reports and laboratory notebook.

Work Placement(s)

No

Syllabus

Theoretical

Introduction to the study of Instrumental Methods of Analysis

Basic notions of analytical instrumentation

Calibrations methods

Spectrophotometric methods

Molecular Ultraviolet and Visible absorption

Molecular Fluorescence

Atomic absorption and emission

Electroanalytical methods

Potentiometry

Voltammetry

Chromatographic methods

Liquid chromatography (HPLC)

Gas chromatography (GC)

Laboratory

Spectrophotometric methods

UV-Visible spectrophotometry: Register of a spectra and quantification of different compounds and drugs.

Fluorescence spectrophotometry: Evaluation of the characteristics of a Fluorescent chemical system and quantification of different compounds and drugs.

Atomic absorption and emission: Quantification of elements (e.g. Zn and K)

Electroanalytical methods

Potentiometry: Direct (pH and fluoride) and indirect determinations (acid/base titrations)

HPLC-Determination of parabens and

GC- Quantificação of ethanol in beverages

Head Lecturer(s)

Ricardo António Esteves de Castro

Assessment Methods

Assessment
Continuous Evaluation : 10.0%
Theoretical-practical test: 20.0%
Exam: 70.0%

Bibliography

Skoog, D.A., Holler, E. J., Crouch, S. R., Principles of instrumental analysis.

7th ed. Thomson Brooks/Cole. 2017.

Skoog, D.A., Holler, E. J., Crouch, S. R., Principles of instrumental analysis.

6th ed. Thomson Brooks/Brooks/Cole. 2007.

Harris, D.C. Quantitative chemical analysis.

9th ed. New York: W. H. Freeman. 2018.

Harris, D.C. Quantitative chemical analysis.

8th ed. New York: W. H. Freeman. 2011.