Analytical Technologies

Recommended Prerequisites

The fundamental base of knowledge must be acquired in the disciplines of physics, chemistry and analytical chemistry. It is also essential to have a good understanding of mathematical calculus and statistics. The inorganic chemistry, organic and physical-chemistry knowledge are complementary.

Teaching Methods

In the theoretical teaching the teacher delivers formal lectures (PowerPoint). The students work in groups, guided by the teacher through the experimental process, in a cooperative learning base. In the classroom the students are encouraged to participate actively in practical-laboratorial classes.

In the evaluation there will be a final written exam about the theoretical teaching (15/20) and a final written test about the practical-laboratory teaching (5/20) that encompasses the fundamentals of practical teaching, laboratory and practical aspects of the implementation of laboratory work

Learning Outcomes

The focus of this curricular unit is the study of the instrumental methods of analysis most used in the field of the Bioanalytical Sciences. It’s expected that the students:

-Understand the chemical and physical principles underlying the instrumental methods of analysis studied.

-Understand the potential and limitations of the instrumental methods studied in solving analytical problems;

-Acquire the basics of analytical instrumentation and its analytical performance characteristics;

-Select the most appropriate analytical methods to analyse a given analyte and matrix:

-Learning to operate the analytical instruments, calibrate and analyse the data from the experiments:

With the practical and laboratory classes it’s expected that the students will be able to:

-Execute with skill and efficiency the lab work;

-Follow rules of safety and environmental protection;

-To analyse and present laboratory results accurately, clearly and concisely;

-Prepare reports and laboratory notebooks.

Work Placement(s)

No

Syllabus

THEORETICAL

Spectrophotometric methods

- Instrumentation

- Analytical applications:

     - Molecular absorption spectroscopy (UV / VIS)

     - Luminescence

     - Fluorescence

     - Chemiluminescence

Infrared spectroscopy (FTIR)

Thermal Analysis (DSC, TG, PLTM)

X-Ray Diffraction (SXD and XRPD)

Chemical Sensors and Biosensors

PRACTICAL

Spectrophotometric determination of nitrite by the Griess reagent

Simultaneous determination of caffeine and acetylsalicylic acid in tablets of Melhoral®

Quantification of salicylic acid in aspirin tablets by UV-Vis spectrophotometry derivative

Spectrofluorimetric determination of propranolol hydrochloride in tablets of Inderal®

Determination of stability constant of complex association of Riboflavin with Caffeine

Quantification of H₂O₂ in commercial hydrogen peroxide by chemiluminescence

Study of polymorphism of pyrazinamide by FTIR

Study of polymorphism of paracetamol by DSC.

Head Lecturer(s)

Rui Manuel Silva Gomes Barbosa

Assessment Methods

Assessment
Other: 25.0%
Exam: 75.0%

Bibliography

Skoog, D.A.; Holler, T.A., Crouch, S. R. Principles of instrumental analysis. 6 ed. Brooks/Cole. 2007.

Harris,D.C., Quantitative chemical analysis. 6 ed. New York: W. H. Freeman. 2010.

H. H. Willard, Instrumental Methods of Analysis. 7 ed. Belmont: Wadsworth Publishing Company. 1988.

Schwedt, G.. The essential guide to analytical chemistry. Chichester: John Wiley & Sons. 1999.

R. Kellner, Analytical chemistry: the approved text to the FECS curriculum analytical chemistry. Weinheim: Wiley-VCH. 1998.

Monk, P., Fundamentals of Electroanalytical Chemistry, Jonh Wiley & Sons, 2001.

Craig. D.Q.M. Thermal Analysis of Pharmaceuticals. CRC press 2007.

Brown, M.E. Introduction to thermal analysis. Kluwer Academic Publishers, 2001.

Watson, David G.; Pharmaceutical Analysis: A Textbook for Pharmacy Students and Pharmaceutical Chemists. 3 ed. Churchill Livingstone Elsevier, 2012.