Metrology and Analytical Validation

Recommended Prerequisites

Relevant curricular units:
-    Laboratory Methodologies (1st Y/1st S)
-    Analytical Chemistry (1st Y/2nd S)
-    Instrumental Analysis (2nd Y/1st S)
Recommended foundation knowledge: general chemistry, organic chemistry, analytical chemistry, instrumental analysis methods.

Teaching Methods

-    Lectures, with incentive to the students active participation, by stimulating critical thinking;
-    Practical laboratory lessons.

Learning Outcomes

-  To give students the required metrological foundations and concepts necessary to perform routine work in an analytical laboratory, giving them the ability to correctly execute various relevant metrological assays;

-  To develop concepts related to strategy and implementation of analytical validation, Good Laboratory

Practice Application, study stages, analytical methods, critical points evaluation, report and protocol making, and revalidation, according to the required guidelines.

Work Placement(s)

No

Syllabus

1.    Metrology: what is it;
2.    History of metrology;
3.    International metrology vocabulary;
4.    Portuguese institute for quality, and its role in metrological control;
5.    International system of units;
6.    Measurement uncertainty;
7.    Correct use and calibration of micropipettes: importance and verification;
8.    Importance and verification of the calibration of commonly used laboratory instruments;
9.    Analytical validation of analytical methods: fundamental concepts and terminology;
10.    Validation parameters: selectivity, specificity, sensibility, detection and quantification limits, precision, accuracy, and robustness; stability;
11.    Acceptability criteria;
12.    Certified materials;
13.    Reference method;
14.    Analytical revalidation;
15.    European guidelines and regulations;
16.    Application in different areas: practical cases.

Head Lecturer(s)

Ana Teresa Sanches Silva

Assessment Methods

Assessment
assiduity: 5.0%
Laboratory work or Field work: 15.0%
Research work: 15.0%
Frequency: 65.0%

Bibliography

Decisão Comissão CE/657/2002-Desempenho de métodos analíticos e interpretação dos resultados
Vocabulário Internacional de metrologia (2008) ed IPQ
Vocabulário Internacional de metrologia Legal (2009) ed.
José Henrique e col. Química Nova, 2002vol25:111.
Guia para quantificação de incerteza em ensaios químicos (publicação PAC) 2007.
Metrologia Industrial 2011, Pedro Guedes ed ETEP.