Analytical Toxicology

Recommended Prerequisites

It takes adequate knowledge in the areas of Chemistry, Anlytical Chemistry, Inorganic Chemistry, Biochemistry, Pharmacology, Physiology, Statistics, Biology, Ecology and instrumental methods of analysis.

Teaching Methods

The unit has a theoretical and a laboratory component. The contents are mainly exposed using a visual media. Although, expository, the critical discussion of the addressed issues is encouraged. In the lectures the contents are mainly exposed using a visual media, promoting the interaction of the students. Analytical methodologies are performed based on isolation, identification and quantification of toxic agents, with emphasis on biological samples and their treatment. On the other hand, the experimental illustration of some general concepts of toxicity is shown.

Learning Outcomes

The course aims to provide students with basic knowledge of toxicology. In the end, the student must acquire basic and specific knowledge in the field of Toxicology; Apply the acquired analytical instrumentation basics; Identify and / or quantify toxic agents involved in occupational, environmental, drug, food or social toxicology, including forensic toxicology and doping; Know how to choose the most appropriate sample for a toxicological analysis; Select the most appropriate analytical method to analyze the toxicant and treat its matrix Perform and organize a complete analytical procedure: sample collection, sample preparation, analytical method of identification and quantification, correct interpretation of the results obtained; Analyze and interpret the results in various areas of Toxicology rigorously; Comply with safety and personal / environmental protection rules and regulations.

Work Placement(s)

No

Syllabus

Theoretical Program:

Toxicology: general principles. Toxic Disposal. Biotransformation of xenobiotics. Toxic to xenobiotic responses. Mechanisms of toxicity. Toxic agents.Clinical Toxicology and Analytical Toxicology / Forensics.Environmental Toxicology. Toxicity assessment: Toxicity tests. Risk assessment.Biomarkers of exposure, effect and susceptibility.General treatment of poisoning.

Analytical Toxicology: Role of the toxicological analysis laboratory in Clinical, Forensic and Industrial or Labor Toxicology. Analytical implications derived from toxicokinetic aspects, relevant to the biotransformation process. Samples, Toxicological analysis, instrumental techniques used in toxicological analysis.

Laboratory Program:

Standards and safety rules in a laboratory. Protocols for isolation, identification and quantification of different toxicants (barbiturates, arsenic, hydrocyanic acid and cyanides, methyl alcohol, fluorine, drugs, pesticides). Spot and screening tests.

Head Lecturer(s)

Isabel Rita Rebelo Ferreira Barbosa

Assessment Methods

Assessment
Exam: 100.0%

Bibliography

Casarett and Doull's Toxicology - The basic science of poisons. Curtis D. Klaassen, Mary O. Amdur; John Doull. 6th ed. McGraw-Hill. New York, 2013

Jungreis, E., Spot test analysis: clinical, environmental, forensic and geochemical applications, John Wiley & Sons, Inc. 2ªEd., 1997

Frederik P.Smith, Jay A, Handbook of forensic drug analysis. Siegel Editor, Elsevier Academic Press, 2005

Flanagan, R.J., Taylor, A., Watson, I.D., Whelpton, R., Fundamentals of analytical toxicology, John Wiley & Sons, 2007

Moreau, R.L.M., Siqueira, M.E.P.B., Toxicologia analítica, Ciências Farmacêuticas, Editora Guanabara Koogan S.A., 2008

Repetto, M. y G., Toxicología Fundamental, Ed. Díaz de Santos, 4ªEd, 2009

Ricardo Jorge Dinis-Oliveira, Félix Dias Carvalho, Maria de Lourdes Bastos. Toxicologia Forense, Editora Pactor, 2015

Ricardo Jorge Dinis-Oliveira, Félix Dias Carvalho, Maria de Lourdes Bastos. Toxicologia Fundamental, Editora Lidel, 2018.