Molecular Biology of Cancer
1
2021-2022
02010549
Cellular and Molecular Biology
English
Face-to-face
SEMESTRIAL
6.0
Elective
2nd Cycle Studies - Mestrado
Recommended Prerequisites
BSc in Life Sciences (Biology, Biochemistry, Pharmacy), or minor in Biology. English spoken and written.
Teaching Methods
Lectures
Practical lectures
Laboratory classes.
Learning Outcomes
Knowledge of the molecular and cellular mechanisms underlying the carcinogenic process, as well as the foundations of the strategies used in the treatment of the disease.
Work Placement(s)
NoSyllabus
The carcinogenic process: theories
Cancer onset and proliferation
Mutations, epimutations;
Oncogenes, oncogene addiction;
microRNAs, siRNAs and riboswitches;
Survival pathways;
Metabolic reprogramming
Cellular counterattack
DNA repair mechanisms;
Tumor suppressors;
Cellular senescence.
Tumor outgrowth
Tumor stroma dynamic interactions;
Paracrine, mechanical, hormonal interactions;
Immune system and adipose tissue roles;
Angiogenesis
Metastases formation theories
Tumor dormancy;
Random and genetically determined processes;
Pre-metastatic niche
EMT & MET
Concept;
Molecular players and pathways;
Wnt/TGF-b/Notch/Hedgehog pathways interplay
Cancer stem cells
Features;
Emergence;
Therapeutic implications
Tumor biobanks
Biopsies collection;
Establishment of primary cultures, propagation and characterization of the cultures
In vitro studies
Immunocytochemistry;
FACs;
Arrays;
Chemo & Radioresistance
In vivo studies
Xenografts, orthotopic models
Humanized models;
Advantages and disadvantages
Head Lecturer(s)
Maria Carmen Martins de Carvalho Alpoim
Assessment Methods
Assessment
Laboratory work or Field work: 15.0%
Other: 30.0%
Exam: 55.0%
Bibliography
Robert Weinberg. The Biology of Cancer. Taylor & Francis, Inc., 2006.
Scientific reviews and original papers